Preliminary ethnobotanic screening in HaCaT cells and skin explants of medicinal plants from the Wadi Araba region in Jordan

Preliminary ethnobotanic screening in HaCaT cells and skin explants of medicinal plants from the Wadi Araba region in Jordan

 לצפייה במאמר בעמוד חדש לחץ כאן

 

Medicinal plants have been traditionally used to address and treat a variety of pathologies including skin diseases. Six medicinal herbs, grown in Jordan, were evaluated for their ability to reduce UVB-induced skin damage, a major contributor to erythema, skin aging and cancer formation. Ethanolic, acetonic and aqueous extracts were prepared from the stems and leaves of the plants. Human keratinocyte HaCaT cells were incubated without or with the different extracts for 24 hr, exposed to UVB irradiation and taken for viability and apoptosis assessment by resazurin and caspase-3 determinations, respectively. Selected extracts were further evaluated by the DCFDA method (ROS production measurement) and on pig and human skin explants. From the 18 extracts screened, only Citrillus colocynthis extracts showed significant protective properties against UVB-induced apoptosis. UV protective activity was found in the water extracts of both stem and leaf preparations of the plant. UV protection was independent of ROS production. Importantly, Citrillus colocynthis extracts were also able to attenuate the photodamage in both ex vivo skin models used in this study. Therefore, the current preliminary screening indicates that Jordanian medicinal plants might be valuable sources for novel skin care compounds. This study also suggests that Citrillus colocynthis extracts exhibit significant photoprotection properties and may attenuate the deleterious effects of UVB radiation.

תאריך פרסום: 
ספטמבר, 2015
Pages: 
66-74

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