Natural products have long been used for skincare and dermo-cosmetic topical formulations. Of particular interest are the unique phytochemical and properties of marine-based extracts and compounds. Both Dunaliella salina and Haloferax volcanii have been shown to possess several health-promoting properties. However, the impact of the extracts on air-pollution-induced damage and wound healing had not been elucidated, as well as their compatibility and possible synergistic actions. Thus, HaCaT human keratinocytes cells were treated without or with increasing concentrations of Dunaliella salina extract, Haloferax volcanii extract, and a combination of both. Their ability to prevent induction of radical formation and skin inflammation by air pollution (diesel particulate matter, DPM) was monitored. In addition, their impact on wound closure and the scavenging capacity of the extracts were determined. DPM markedly enhanced cellular reactive oxygen species (ROS) formation and secretion of the inflammatory cytokine interleukin-8 (IL-8). Treatment with Haloferax volcanii extract reduced ROS in a dose-dependent manner, whereas Dunaliella salina had no significant effect. Importantly, the combination of both showed synergistic activity. Similar results were obtained in the quantification of IL-8 secretion. In the wound healing model, both extracts were ineffective as individual treatments, and their combination markedly enhanced wound closure. Finally, the acellular scavenging results correlate with the in vitro data, suggesting that the synergistic action is due to the antioxidant properties of the combinations. The results suggest that a combination of Dunaliella salina and Haloferax volcanii can be used as an effective skincare complex with several applications. Thorough phytochemical analysis should be performed in order to pinpoint the active medicinal compounds underlining these in vitro activities, as well as a clinical validation.
מאת: O. Raz, N. Ogen-Shtern, N. Kuchina, G. Cohen
תאריך פרסום: ספטמבר, 2021
מהדורה: 13 (3)
סוג מאמר: מאמר מחקר
קישור להורדה: journal13-3-1.pdf